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Cdh16-cre

WebOct 5, 2024 · To explore the function of CDK12 in the kidney, we generated a novel CDK12 flox/flox line harboring loxP sites flanking exon 4 of the Cdk12 gene. By breeding with cdh16-Cre transgene mice, in which Cre expression is limited to the renal tubules, including Bowman’s capsule, proximal tubule, loop of Henle, distal tubule, and the collecting duct, … WebNov 7, 2016 · To detect conversion towards tubule cell identity, we crossed mice harbouring Cre-recombinase under control of the renal tubule-specific cadherin-16 promoter (Cdh16/Ksp-Cre (kidney-specific ...

Epithelial-specific Cre/lox recombination in the …

WebMay 15, 2024 · By breeding with cdh16-Cre transgene mice, in which Cre expression is limited to the renal tubules, including Bowman’s capsule, proximal tubule, loop of Henle, distal tubule, and the collecting duct, we … Web012237 B6.Cg-Tg(Cdh16-cre)91Igr/J These Ksp1.3/Cre transgenic mice express Cre recombinase under the control of the mouse cadherin 16 ( Cdh16 or Ksp1.3) promoter. Cre recombinase expression is detected in epithelial cells of the developing kidney and genitourinary tract and in the renal tubules of adult mice. This mutant mouse strain may … footes sheds ny https://bdraizada.com

Tubular-specific CDK12 knockout causes a defect in …

WebJul 1, 2014 · Epithelial specific knockout of Invs in Invs flox/flox;Cdh16-Cre mutant mice resulted in renal cyst formation and severe stromal fibrosis, while Invs flox/flox;Foxd1-Cre mice, where Invs is deleted in stromal cells, displayed no observable phenotypes up to the young adult stage, highlighting a significant role of epithelial-stromal crosstalk. WebJun 22, 2024 · Cdh16, also known as kidney specific protein (Ksp), is expressed in kidney distal nephrons including the loop of Henle, distal convoluted tubules, and collecting … WebApr 7, 2024 · EZH2 conditional knockout mice were generated by breeding our EZH2 fl/fl mice with Cdh16-Cre mice to obtain Cdh16-Cre +: EZH2 fl/fl mice (EZH2-cKO) and Cdh16-Cre −: EZH2 fl/fl mice (EZH2-WT). footes timaru

Tubular-specific CDK12 knockout causes a defect in …

Category:CDH16 Gene - GeneCards CAD16 Protein CAD16 Antibody

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Cdh16-cre

Figures and data in Modelling TFE renal cell carcinoma in mice …

WebCre recombinase expression is detected in epithelial cells of the developing kidney and genitourinary tract and in the renal tubules of adult mice. This mutant mouse strain may … Please use this form to enter mouse-related technical questions, comments, … WebTo this end a 1.4-kb promoter fragment of the kidney-specific cadherin gene (KspCad) was cloned upstream of a tamoxifen-inducible Cre recombinase (CreER(T2)) encoding sequence. Expression and activity of Cre was evaluated using reverse transcriptase polymerase chain reaction (RT-PCR) analysis and by crossbreeding to Z/EG reporter mice.

Cdh16-cre

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WebB6.Cg-Tg (Cdh16-cre)91Igr/J Stock No: 012237 Protocol 25023: Standard PCR Assay - Tg (Cdh16-cre) Version 2. 2 Notes Validation/LC Conversion data attached to rec 128142 … WebSep 26, 2016 · An extensive molecular and biochemical characterization of kidneys, as well as of primary kidney cells, derived from these mice revealed a significant hyper …

WebSep 26, 2016 · (A) Tfeb, Cyclin D1, Myc and Axin2 mRNA levels in kidneys from Cdh16 Cre and Cdh16 Cre::Tfeb fs mice treated with vehicle or PKF118-310. Values are shown as … WebTg (Cdh16-cre/ERT2)F427Djmp. Mutation details : The transgene consists of 1.4 kb fragment of the murine Cdh16 5' promoter region and a tamoxifen-inducible form of cre recombinase. Inducible Cre activity is seen in renal tubular epithelial cells and collecting ducts. ( J:110146 )

WebMar 1, 2024 · Cdh16 (Cadherin16, or Ksp-cadherin) is strongly expressed in epithelia of nephron tubules and collecting ducts in rabbit, mouse and human. Transgenic mice with …

WebMar 1, 2024 · Cdh16 (Cadherin16, or Ksp-cadherin) is strongly expressed in epithelia of nephron tubules and collecting ducts in rabbit, mouse and human. Transgenic mice with exogeneous gene driven by Cdh16 promoter (e.g. Ksp-Cre) is widely used in basic research of nephrology. To determine whether the pig Cdh16 gene could be utilized to generate …

WebKsp-cadherin is a unique, tissue-specific member of the cadherin family of cell adhesion molecules that is expressed in tubular epithelial cells in the kidney and developing … elevated aquarium lightingWebThis gene is a member of the cadherin superfamily, genes encoding calcium-dependent, membrane-associated glycoproteins. Mapped to a previously identified cluster of … elevated architectureWebAug 1, 2002 · We took advantage of well-characterized Cdh16 Cre mice, in which the Cre recombinase is expressed in the epithelial lining of proximal tubules, collecting ducts, loops of Henle, and distal tubules ... footes tireWebPkd1 fl/fl; Cdh16-Cre mice were generated by crossing Pkd1 fl/fl mice with Cdh16-Cre mice. Pkd1 fl/fl; Cdh16-Cre mice were then crossed with A-CREB transgenic mice. Both sexes were used in the kidney-specific mouse model, and mice were euthanized at P7. Five pairs of kidneys were collected for tissue section analysis and the rest were used for ... elevated archery blindsWebCdh16 Cre :: Tfeb fs animals, they were detected starting from P90 with an incidence of 23% (5 cases out of 21 Cdh16 Cre ::Tfeb fs mice older than 3 months). elevated archery wrapsWebEZH2 conditional knockout mice were generated by breeding our EZH2 fl/fl mice with Cdh16-Cre mice to obtain Cdh16-Cre +: EZH2 fl/fl mice (EZH2-cKO) and Cdh16-Cre −: EZH2 fl/fl mice (EZH2-WT). Our data showed that EZH2-cKO or inhibition of EZH2 with 3-DZNep significantly alleviated kidney damage and reduced serum creatinine and BUN in … elevated archery shooting platformWebJun 28, 2024 · Cdh16-Cre-: HDAC4 fl/fl (HDAC4-WT) and Cdh16-Cre +: HDAC4 fl/fl (HDAC4-KO) mice were killed 7 days after sham operation or UUO. (A) Micrographs of vimentin and p-Histone H3(Ser10) immunofluorescence staining; box at the lower right of vimentin/WT/UUO is a micrograph twice the size of the original partial position (Original … footes tire sayre